Selective binding of cholesterol by recombinant fatty acid binding proteins.
نویسندگان
چکیده
منابع مشابه
Polyene fatty acid interactions with recombinant intestinal and liver fatty acid-binding proteins. Spectroscopic studies.
Binding and proximity relationships of fatty acids with recombinant rat liver fatty acid-binding protein (L-FABP) and intestinal fatty acid-binding protein (I-FABP) were studied with absorption and fluorescence spectroscopy. Protein aromatic amino acids were examined in the absence and presence of bound fatty acid. Second derivative absorbance spectroscopy of the apo- and holoproteins suggested...
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Les protkines de liaison ou de transfert des acides gras sont prCsentes dans les fluides intraet extra-cellulaires de la plupart des organismes. Elles jouent un r61e dans le transport ou le ciblage des acides gras, mais peuvent kgalement agir en interaction avec different processus par la modulation de la concentration de la liaison libre. Quelques protkines de liaison des acides gras sont asse...
متن کاملInteraction of fatty acids with recombinant rat intestinal and liver fatty acid-binding proteins.
Intestinal enterocytes contain two homologous fatty acid-binding proteins, intestinal fatty acid-binding protein (I-FABP)2 and liver fatty acid-binding protein (L-FABP). Since the functional basis for this multiplicity is not known, the fatty acid-binding specificity of recombinant forms of both rat I-FABP and rat L-FABP was examined. A systematic comparative analysis of the 18 carbon chain len...
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Fatty acid binding protein (FADP) is an attractive vaccine candidate and a drug target for controlling fasciolosis caused by Fasciola gigantica in ruminants. The cDNA encoding F. gigantica FADP was amplified by polymerase chain reaction and expressed in Escherichia coli as a recombinant protein using a prokaryotic expression system. The protein was purified to homogeneity by nickel chelating af...
متن کاملQuantitation of hepatic fatty acid-binding proteins by post-chromatographic ligand binding assay.
A new procedure for the detection and quantitation of small molecular weight cytosolic fatty acid-binding proteins (FABP) in chromatographic fractions is described. Aliquots of the fractionated cytosol are incubated with radiolabeled palmitate and the unbound fatty acid ligand is quickly removed by addition of a dextran-gelatin-coated charcoal suspension. Quantitation of the FABP is accomplishe...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)47356-0